Cladonia is a widespread genus of lichenized Ascomycetes whose knowledge is fragmentary in Italy. There, many records are dispersed in floristic studies, while specific research targeted on this lichen group is almost missing. As a first contribution to the knowledge of the Cladonia biota of Tuscany, the annotated list of the species recorded in Monte Ceceri (Fiesole, province of Florence) is reported. Eleven species are listed; among them, C. ciliata likely became extinct since the only historical record, while C. peziziformis and C. rei are reported for the first time from Tuscany. Similarly to other areas recently studied in northern Italy, also in Monte Ceceri the occurrence of Cladonia-rich terricolous lichen communities is related to the persistence of fragments of open dry habitats, which are threatened by vegetation encroachment. This case study is a first step towards a better knowledge of terricolous lichen communities of lowlands of central Italy, which provides information useful for the conservation of these overlooked organisms.