A re-examination of herbarium material together with some new finds from Southern Italy (the regions Basilicata, Calabria, Campania, Puglia and Sicilia) resulted in a list of 39 lichenicolous fungi (and 8 species of lichenicolous lichens). Six of them are new to Italy [Arthonia caerulescens (Almq.) R.Sant., Arthonia protoparmeliae Etayo, Arthonia xanthoparmeliarum Etayo, Tremella diploschistina Millanes & al., Verrucoccum coppinsii V. Atienza & al. and Zwackhiomyces polischukii Darmostuk & Khodos.] and 13 new to the respective region. The species Lichenotubeufia calabrica Brackel, Sphaerellothecium nimisii Brackel & Puntillo and Stigmidium hypogymniae Brackel are described as new to science